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   To determine if their test strain was behaving like the others, they first ran stress tests on them. They tested the bacteria's response to the physical barrier, ultraviolet light, and antibiotics. The addition of a physical barrier led to the transition of bacteria from one layer to a multilayer layer, followed by an increase in cell density and the formation of multilayer islands near the barrier. Later, wrinkles formed on the islands near the barrier in the place where they began to appear initially.
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   When they applied ultraviolet light to the swarm, they again observed a drop in cell speed and an increase in density. And after the scientists added a large dose of the antibiotic kanamycin, the bacterial cells formed a biofilm. The researchers then developed a strategy to combat this bacterial biofilm. They added kanamycin to the environment of a new batch of swarming bacterial cells and watched as the biofilm began to form. They then re-injected the antibiotic at a much higher dose than the first, just before the biofilm formation was complete. As a result, the partially formed biofilm was destroyed and bacterial cells died. This shows that antibiotic-resistant bacteria lose their resistance to antibiotics when they undergo a phase transition, right before going into the biofilm, where they will become much more resistant. Thus, with the correct administration of antibiotics, the bacteria can be attacked in their most vulnerable state and destroyed. The researchers believe that similar transitions from swarm to biofilm occur in other bacterial species. Their research may pave the way for finding more effective ways to control clinically relevant bacteria. For example, Salmonella enterica, which spreads into the bloodstream and is transmitted through contaminated food. Or Pseudomonas aeruginosa with multiple drug resistance, which after surgery causes infections in the blood, lungs (pneumonia) and other parts of the body and spreads in hospitals.
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   Stress often causes bacteria to form biofilms. Stress can manifest as a physical barrier, ultraviolet light, or a toxic substance such as antibiotics. The formation of these biofilms takes from several hours to days, and they can be of different shapes, sizes, colors, and textures depending on the type of bacteria. Being in a biofilm state protects them from harmful substances in the environment - biofilms have a unique outer wall with different physical and chemical properties than their individual cells. They can coordinate metabolism, slow down their growth, and even form an impenetrable barrier of wrinkles and creases. This is one of the ways they achieve high antibiotic resistance. Researchers from the United Kingdom recently studied the transition of the Hay Bacillus bacterium from a free-moving swarm to a biofilm as a defense mechanism and published what they did to combat its antibiotic-resistant properties in eLife.
   
   To determine if their test strain was behaving like the others, they first ran stress tests on them. They tested the bacteria's response to the physical barrier, ultraviolet light, and antibiotics. The addition of a physical barrier led to the transition of bacteria from one layer to a multilayer layer, followed by an increase in cell density and the formation of multilayer islands near the barrier. Later, wrinkles formed on the islands near the barrier in the place where they began to appear initially.
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   When they applied ultraviolet light to the swarm, they again observed a drop in cell speed and an increase in density. And after the scientists added a large dose of the antibiotic kanamycin, the bacterial cells formed a biofilm. The researchers then developed a strategy to combat this bacterial biofilm. They added kanamycin to the environment of a new batch of swarming bacterial cells and watched as the biofilm began to form. They then re-injected the antibiotic at a much higher dose than the first, just before the biofilm formation was complete. As a result, the partially formed biofilm was destroyed and bacterial cells died. This shows that antibiotic-resistant bacteria lose their resistance to antibiotics when they undergo a phase transition, right before going into the biofilm, where they will become much more resistant. Thus, with the correct administration of antibiotics, the bacteria can be attacked in their most vulnerable state and destroyed. The researchers believe that similar transitions from swarm to biofilm occur in other bacterial species. Their research may pave the way for finding more effective ways to control clinically relevant bacteria. For example, Salmonella enterica, which spreads into the bloodstream and is transmitted through contaminated food. Or Pseudomonas aeruginosa with multiple drug resistance, which after surgery causes infections in the blood, lungs (pneumonia) and other parts of the body and spreads in hospitals.
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    To write a diploma easier, you need to find a middle ground: the topic should not be "hackneyed" or too rare.
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    Otherwise, it will be difficult to find aspects that are not covered by anyone and, on the contrary, to select materials for development, analysis, argumentation, comparison.
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    The next stage is complex and consists in defining goals, objectives, research methods, selection and systematization of relevant literature and parallel drawing up of a thesis plan, which is a preliminary outline of the structure and content of future student scientific work. When familiarizing with the sources of information, one should take into account their novelty. The periods of obsolescence of educational literature are established by order of the Ministry of Education and Science No. 1623 of April 11, 2001: for a general humanitarian, socio-economic profile and special disciplines - five years; for general professional, natural science, mathematical subjects - ten years. The measures of relevance do not relate to sources of in-depth fundamental study of the issue. It does not stipulate the order and terms after which research publications: articles, dissertations, reports, etc., are considered outdated, but university regulatory controllers, when checking their thesis for compliance with standards, are almost always guided by the requirements set for textbooks. For informational purposes, no one forbids using them, but in order to avoid sending the finished diploma for revision, it is better to include only works no older than five years in the list of used literature. In January 2017, the order of the Ministry of Education and Science entered into force No. 1651, canceling the effect of orders No. 1623 and No. 133, which regulated the provision of university libraries with scientific and educational literature (it was in them that the terms of its obsolescence were prescribed). The new document aroused a lot of questions from the experts of the academic community, and the official answers of the department to them did not clarify the situation, so teachers still prefer to adhere to the old standards, determining the "expiration date" of the sources used to write the thesis. Algorithm of writing The scheme of work on a diploma is standard for all students - according to a single algorithm, graduates who write both project and research FQPs go to future defense. First of all, you need to decide on a supervisor, then choose a topic. It is approved before the start of the pre-diploma practice, to which the student is sent with the assignment for the FQP signed by the head of the department.
    The structure of the thesis According to the generally accepted rules for writing a diploma, its main part should consist of three chapters: First (at least three paragraphs). Written on the basis of a synthesis and analysis of theoretical information gleaned from selected sources; reveals the central concepts, the essence of a phenomenon or process, different points of view on the object and subject of research. Second (at least three subsections). Assigned to factual material and analysis of the problem; contains statistical information with quantitative and qualitative characteristics of the phenomenon under consideration, a statement of the identified trends and shortcomings in its state and development. Third (the minimum number of paragraphs is three). Dedicated to developing a solution to a problem. His diploma student proposes on the basis of the second chapter with scientific justification, for which the theoretical provisions of the first are involved. The chapters should be approximately equal in length. At the final stage, a conclusion, an introduction, a final list of used literature are drawn up and a block with annexes is completed. How to properly draw up a thesis In student memos and on their official websites, all universities must post detailed methodological recommendations containing requirements for a diploma. They indicate: volume - how many pages in the diploma should be; structure - how many chapters to divide the study, what to write in the introduction and conclusion; what materials should be included in the attachments and how to number them; technical regulations - where to put down, from which sheet to start pagination, what font to use for text and headings, how to indent the paragraph and margins, line spacing; the number of sources in the bibliographic list; permissible percentage of uniqueness when checking for plagiarism. Most universities and institutes include in methodological collections: a template for the title page; forms of reviews, annotations, reviews; sample bibliography; examples of the design of references, footnotes, formulas, tables, figures, diagrams, photos. It remains only to carefully check with the university standard and follow the recommendations, since the normative control will be meticulously carried out precisely according to the points of the manual, and only then relying on GOSTs. Average requirements for a thesis: Volume - 50-70 pages, excluding attachments and, as a rule, bibliography. The number of sources is at least 40, and 70% are publications not older than five years. The bibliographic list is drawn up in accordance with GOST 7.80-2000, 7.1-2003, 7.0.5-2008, 7.0.1-2011. Times New Roman in black, 14th for text, 15th for subheadings, 16th for headings. The interval is 1.5. Margins - at least 2 cm to the left, bottom and top; 1.5 cm - on the right. Printing is only one-sided. The standard of uniqueness of the diploma: humanities - 80%, exact - 75%.
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